PCR Reagents

Product Details:

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Number of bands: 11
Range: 100 - 1,500 bp
Concentration: 100 μg / ml
Package: 50μg / 500μl
Recommended Load: 5 μl / well ( ** Containing orange G & xylene cyanol FF as tracking dyes. )
Source : PCR products and double-stranded DNA digested with appropriate restriction enzymes are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10mM EDTA.

Storage :

Stable for up to 6 months at 25°C.
Stable for up to 12 months at 4°C.
Stable for up to 24 months at -20°C.

Additional Information:

• Item Code: BIO-HELIX-DM001-R500
• Packaging Details: 100 bp DNA ladder 500 microlitre volume

Product Details:

2X PCR SuperMix is a cocktail of calculated amounts of Mg++, dNTPs, and recombinant Taq DNA Polymerase for highly efficient amplification of nucleic acid templates by the polymerase chain reaction (PCR). 2X PCR SuperMix is supplied at a 2X concentration that allows the easy calculation of its final concentration for adding the primer and template to the mixture. The supplied reagent is sufficient for 100 amplification reactions of 50 μL each. The 2X PCR SuperMix is very stable, and no detectable reduction of PCR performance or enzyme activity is observed after storage for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance or activity.??? Storage and Heat Endurance: 21 days in 37°C stable.
??? Super high speed : Complete 2 kb in 60 seconds (30b ~ 50b/sec in extension).
??? High sensitivity: Low copy number of template amplification.
??? Amplification of long targets up to 5kb from Lambda DNA.
??? Best balance between performance and value.

Additional Information:

• Item Code: BIO-HELIX-MB200-P100

Product Details:

Taq DNA Polymerase is purified from E. coli. expressing a Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and a 5′ → 3′ exonuclease activity but lacks a 3′ → 5′ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer. Taq Polymerase is recommended for use in routine PCR reactions. The buffer system is optimized for high specificity and guarantees minimal by-product formation. We supplied Taq Polymerase with appropriate buffers. Usually 1-1.5 u of Taq DNA Polymerase are used in 50 µl of reaction mix. Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purified), higher amounts of Taq DNA Polymerase (2-3 u) may be necessary to obtain a better yield of amplification products.

Additional Information:

• Item Code: MB101-0500