Biolinx Labsystems Private Limited
Biolinx Labsystems Private Limited
Mahape, Navi Mumbai, Maharashtra
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Field Flow Fractionation systems

Field Flow Fractionation (FFF) systems

Postnova is Inventor of Field-Flow Fractionation and has 30 years of experience in innovation and excellence of FFF-LS. Prof. Calvin Giddings from University Utah, Salt Lake City, USA invented and patented Field-Flow Fractionation in 1966.

Since then there has been lot of developments and various platforms have been developed for Field-Flow Fractionation.

The unique Postnova FFF Platform can be coupled to MALS, DLS, RI, UV and ICP-MS detectors

We have complete range of modules like detectors and eluent pumps which can be coupled with FFF

  •     Eluent Pumps
  •      Multi-Angle Light Scattering Detector with 21 angles
  •     Refractive Index Detector
  •     ETC. 

Field-Flow Fractionation Systems

Field-Flow Fractionation Systems
  • Field-Flow Fractionation Systems
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AdvantagesUltrahigh resolution and very broad size range for polymers and particles

Field-Flow Fractionation is a family of separation techniques, comprising of various different sub-techniques. All these Field-Flow Fractionation versions utilize the same basic separation principle, but employ different force fields. Depending on the used separation field the technique is called Flow Field-Flow Fractionation, Sedimentation Field-Flow Fractionation, Thermal Field-Flow Fractionation or Split Flow Thin Cell Fractionation (SPLITT).Field-Flow Fractionation is providing fast, gentle and high resolution separations of any particulate matter from 1 nm up to 100 µm in a liquid medium. The sample is separated inside an open flow channel without the presence of any packing or stationary phase inside.

Unique Advantages
  • Ultrahigh resolution and very broad size range for polymers and particles
  • No shear forces and gentle separation especially for biomolecules
  • Direct molar mass and particle size determination without calibration standards
  • Temperature controlled system with patented reusable separation channel
  • No stationary phase or columns as spare part necessary
  • Analytes can be directly fractionated, collected and measure with additional on- and offline analytical methods as fluorescence, EM, AFM, etc.
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Centrifugal Flow FFF System - CF2000

Centrifugal Flow FFF System - CF2000
  • Centrifugal Flow FFF System - CF2000
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Required Analysis Time:Typically 30-90 min: shorter/longer times possible
Software System:Unique NovaFFF
Centrifugal Force/Rotation SpeedFrom 2 up to 2680 g,Maximum of 4900 rpm

The most Advanced System for High Resolution Separation and Fractionation of Nanoparticles! Due to it’s unique design, the CF2000 Centrifugal FFF system offers more flexibility, higher robustness and better performance than traditional particle sizer systems. The CF2000 allows high resolution particle separation and sizing at the same time and consequently sets a complete new standard and offers a real alternative to traditional particle characterization techniques.
  • High Resolution Separations

Centrifugal FFF differentiates itself from the various particle size analyzers, which are available on the market, because of its ability to deliver true high resolution particle size separations.

  • Broad Separation Size Range

The CF2000 technology allows separation of small, medium and large particles at the same time in just one analysis and avoids the discrimination of smaller particles by larges species which is often a limitation in light scattering based techniques.

  • Separation under Native Conditions

Using the unique unpacked and open Centrifugal FFF channel, allows to employ “any” kind of eluent which is ideally suited for the sample particles.

  • Separation of same Size Particles

Because Centrifugal FFF separates by size and density, it is possible to distinguish particles of same size which have different chemical compositions and structures.

  • Flexibility & Interfaceability

The CF2000 Series was developed to be used as a modular FFF system which can be interfaced with existing other detection systems, such as UV, DLS, MALS, SAXS. This way Centrifugal FFF can used as a high resolution size fractionation system, which then can be combined on- or offline with high resolution detection systems, which provides an ultimate 2D view of any sample system.

Technical Specification

Temperature Range:
Typically Room Temperature, Limits: 5°C to 50°C
Measurement Range:
Nanoparticles: 7 nm – 20 µm
Minimum sizes achievable:
Al2O3, MnO2, Fe2O3, Clay, Si Au, Ag, are
12.5 nm, 12.5nm, 12.3 nm, 17.3 nm, 19.9 nm, 7.6 nm, 8.4 nm
Channel Cartridge System:
Special Postnova centrifugal channel system:
Inner Volume: ca. 1.5 mL depending on spacer
Inner Channel Thickness: 250 µm (others available)
Channel Diameter: 180 mm
Connected Detector Systems:
The following detection technologies can be used in FFF:
Dynamic Light Scattering – DLS
Static Light Scattering – MALS
UV/VIS Absorbance
Flow Rate Ranges / Pressure:
Main Flow 0.01 – 5.0 mL/min at channel outlet
Channel Pressure up to 25 bar maximum
Centrifugal Force/Rotation Speed:
From 2 up to 2680 g
Maximum of 4900 rpm
Constant and Linear-Exponential Decay Field
Required Analysis Time:
Typically 30 – 90 min; shorter/longer times possible
Software System:
Unique NovaFFF software platform for complete system control (autosampler, pumps, and detectors), data acquisition (detector signals, pressure, flows) and evaluation as well as reporting functions (static light scattering calculations, standard calibration).
Injection Volumes:
Typically 10 – 100 µL with autosampler injection
Typically 10 – 100 µL with manual injection
Band broadening can occur when too high injection volumes are used.
Injected Sample Mass:
Limiting parameter is channel overloading, which is primarily depending on the channel dimensions. Typical injected sample mass for given channel size is 10 – 100 µg. The maximum sample mass can go up to 500 µg, depending on sample nature.

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Asymmetric Flow FFF- AF2000 MultiFlow FFF

Asymmetric Flow FFF- AF2000 MultiFlow FFF
  • Asymmetric Flow FFF- AF2000 MultiFlow FFF
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seperation upto 10E9 DA molar mass

The most Advanced Flow FFF Platform for Separation of Proteins, Macromolecules and Nanoparticles

Benefits

  • Professional Modular Platform

The new Postnova AF2000 MultiFlow FFF Series was invented to become the first professional modular Flow FFF platform available. It is completely integrated by the NovaFFF single software platform which runs the entire system from autosampler to detectors.

  • Universal Flexible Separator

The AF2000 Series platform is based on the Flow FFF principle, using a crossflow field as driving force for the separation. The AF2000 MultiFlow can be easily used for separation of a broad range of different samples from the area of Biopharmaceuticals, Food-Agro-Cosmetics, Environmental, Chemicals and Nanotechnology.

  • Broad Separation Range

No size exclusion effect is observed, making HTFFF the ideal tool for the separation of large and ultra-large polymers or even nanoparticles.

  • Gentle Separation Conditions

Because of the open channel and the absence of any stationary phase the separation can be performed under the absence of shear forces and stress to the macromolecule.

  • Patented FOCUS Technology

The AF2000 HT incorporates Postnova’s patented FOCUS technology, to ensure a continuous and stable detector flow at any time. The technology provides also a higher sample recovery, higher resolution and sensitivity.

  • Patented Cross Flow System

Postnova is using a patented technology for generating the cross flow inside the FFF channel, based on a double syringe pump. This pump is controlled in a special unique way avoiding any pulsation.

  • Flexibility & Interfaceability

The AF2000 MT series was developed to be used as Stand-Alone system or interfaced with existing HTGPC systems from various manufacturers. This gives the advantage that FFF and GPC can be performed using the same set or detectors, using the same temperature chamber for GPC and FFF.

Types

  • AF2000 AT – Standard Ambient Temp Flow FFF

The AF2000 AT is a non-temperature controlled economical Flow FFF which is ideal for the use in general level research in universities, companies and public scientific centers.

  • AF2000 MT – Advanced Mid Temp Flow FFF

The AF2000 MT is a temperature controlled advanced Flow FFF system, which is ideal for the use in higher level research in university and public research institutions as well as professional research and commercial-industrial applications in the area of product development, manufacturing and quality control.

  • AF2000 HT – Toplevel High Temp Flow FFF
The AF2000 HT is a special temperature controlled toplevel Flow FFF system, which is ideal for the use in research, development and quality control of polyolefins and high temperature macromolecules in universities, public research institutions and industry.
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Gravitational Split FFF- SF2000 G

Gravitational Split FFF- SF2000 G
  • Gravitational Split FFF- SF2000 G
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Brand NameGravitational Splitt FFF- SF2000 G
UsageTo separate, fractionate, purify and to characterize microparticles in one step.
ApplicationCommercial G-Splitt

The SF2000 G is a the first true completely integrated and modular commercial G-Splitt including online optical particle detection. The machine can be used to separate, fractionate, purify and to characterize microparticles in one step.
  • True Preparative Microparticle Fractionation

The SF2000 G allows continuous separation and fractionation of microparticles by size and density. It is possible to collect mg to gram quantities and reach preparative scales.

  • Broad Separation Range

The system is using a gravity field and thus can separate microparticles from about 1 to 300 μm in typical aqueous based carrier solutions. A resolution of 1 μm size difference can be achieved for smaller particles.

  • Gentle Separation Conditions

Because of the open channel and the absence of any stationary phase the separation in Splitt FFF can be performed under the absence of shear forces and stress avoiding particle aggregation.

  • On-line and Off-Line Detection

The SF2000 G system can be easily hyphenated on- and offline to additional advanced detection systems, such as Absorbance (UV/Vis), Fluorescence (FLD), Dynamic Light Scattering (DLS), Optical Particle Detection (XPT), X-Ray, Laser Diffraction and Microscopy.

  • NovaFFF XPT Particle Detection
The SF2000 G has an online coupled Optical Particle Detection system, PN3000 XPT, as back end. This detection system allows to completely characterizing the separated particle size fractions as they elute from the Splitt channel.
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Thermal Flow FFF -TF2000 Polymer Separator

Thermal Flow FFF -TF2000 Polymer Separator
  • Thermal Flow FFF -TF2000 Polymer Separator
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Grade StandardTechnical Grade, Analytical Grade, Bio-Tech Grade
UsageIndustrial, Laboratory
Physical StateSolid, Liquid, Granules

The most Advanced System for Separation of Natural and Synthetic Polymers and Gel Particles. The TF2000 Series incorporates the combined solid know-how and the proven technologies from three decades of leadership in FFF.

  • Separation of Polymers, Gels and Particles

The TF200 allows the simultaneous separation, characterization and fractionation of polymers, gels and particles within one measurement. The channel is reusable and only needs to be cleaned from time to time. There is no consumable which needs to be replaced frequently.

  • Broad Separation Range

No size exclusion effect is observed, making the TF2000 the ideal tool for the separation of large and ultra-large cross-linked polymers.

  • Gentle Separation Conditions

Because of the open channel and the absence of any stationary phase the separation can be performed under the absence of shear forces and stress to the macromolecule. Typical chromatography problems, such as filtering/adsorption effects, shear induced chain degradation and late elution effects can be avoided.

  • Broad Range of Eluents

Because of the absence of the stationary phase, nearly any kind of organic and even some aqueous liquids can be used in Thermal FFF. This allows to easily use also aggressive and otherwise difficult to use eluents such as DMSO for starch analysis etc. There is no need to add salt such as LiBr which is often essential in chromatography to avoid polymer interacting with stationary phases.

  • R&D as well as QC Capability

The TF2000 can be remote controlled, meets and conforms with the most stringent international security regulations ad offers series of automatic security features to allow unattended use. Temperature, pressure, leak and gas sensors are constantly monitoring the system for reproducibly and stable operation even in rough production environments.

  • Flexibility & Interfaceability

The TF2000 series was developed to be used as Stand-Alone system or interfaced with existing chromatographic systems from various manufacturers.

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Abhishek Shrotriya (Marketing Manager)
No. 203, Techno City, Plot No. X5/3
Mahape, Navi Mumbai- 400710, Maharashtra, India



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