Field Flow Fractionation (FFF) systems
Postnova is Inventor of Field-Flow Fractionation and has 30 years of experience in innovation and excellence of FFF-LS. Prof. Calvin Giddings from University Utah, Salt Lake City, USA invented and patented Field-Flow Fractionation in 1966.
Since then there has been lot of developments and various platforms have been developed for Field-Flow Fractionation.
The unique Postnova FFF Platform can be coupled to MALS, DLS, RI, UV and ICP-MS detectors
We have complete range of modules like detectors and eluent pumps which can be coupled with FFF
|Advantages||Ultrahigh resolution and very broad size range for polymers and particles|
|Centrifugal Force/Rotation Speed||From 2 up to 2680 g,Maximum of 4900 rpm|
|Software System:||Unique NovaFFF|
|Required Analysis Time:||Typically 30-90 min: shorter/longer times possible|
Centrifugal FFF differentiates itself from the various particle size analyzers, which are available on the market, because of its ability to deliver true high resolution particle size separations.
The CF2000 technology allows separation of small, medium and large particles at the same time in just one analysis and avoids the discrimination of smaller particles by larges species which is often a limitation in light scattering based techniques.
Using the unique unpacked and open Centrifugal FFF channel, allows to employ “any” kind of eluent which is ideally suited for the sample particles.
Because Centrifugal FFF separates by size and density, it is possible to distinguish particles of same size which have different chemical compositions and structures.
The CF2000 Series was developed to be used as a modular FFF system which can be interfaced with existing other detection systems, such as UV, DLS, MALS, SAXS. This way Centrifugal FFF can used as a high resolution size fractionation system, which then can be combined on- or offline with high resolution detection systems, which provides an ultimate 2D view of any sample system.
Typically Room Temperature, Limits: 5°C to 50°C
Nanoparticles: 7 nm – 20 µm
Minimum sizes achievable:
Al2O3, MnO2, Fe2O3, Clay, Si Au, Ag, are
12.5 nm, 12.5nm, 12.3 nm, 17.3 nm, 19.9 nm, 7.6 nm, 8.4 nm
Channel Cartridge System:
Special Postnova centrifugal channel system:
Inner Volume: ca. 1.5 mL depending on spacer
Inner Channel Thickness: 250 µm (others available)
Channel Diameter: 180 mm
Connected Detector Systems:
The following detection technologies can be used in FFF:
Dynamic Light Scattering – DLS
Static Light Scattering – MALS
Flow Rate Ranges / Pressure:
Main Flow 0.01 – 5.0 mL/min at channel outlet
Channel Pressure up to 25 bar maximum
Centrifugal Force/Rotation Speed:
From 2 up to 2680 g
Maximum of 4900 rpm
Constant and Linear-Exponential Decay Field
Required Analysis Time:
Typically 30 – 90 min; shorter/longer times possible
Unique NovaFFF software platform for complete system control (autosampler, pumps, and detectors), data acquisition (detector signals, pressure, flows) and evaluation as well as reporting functions (static light scattering calculations, standard calibration).
Typically 10 – 100 µL with autosampler injection
Typically 10 – 100 µL with manual injection
Band broadening can occur when too high injection volumes are used.
Injected Sample Mass:
Limiting parameter is channel overloading, which is primarily depending on the channel dimensions. Typical injected sample mass for given channel size is 10 – 100 µg. The maximum sample mass can go up to 500 µg, depending on sample nature.
|seperation||upto 10E9 DA molar mass|
The new Postnova AF2000 MultiFlow FFF Series was invented to become the first professional modular Flow FFF platform available. It is completely integrated by the NovaFFF single software platform which runs the entire system from autosampler to detectors.
The AF2000 Series platform is based on the Flow FFF principle, using a crossflow field as driving force for the separation. The AF2000 MultiFlow can be easily used for separation of a broad range of different samples from the area of Biopharmaceuticals, Food-Agro-Cosmetics, Environmental, Chemicals and Nanotechnology.
No size exclusion effect is observed, making HTFFF the ideal tool for the separation of large and ultra-large polymers or even nanoparticles.
Because of the open channel and the absence of any stationary phase the separation can be performed under the absence of shear forces and stress to the macromolecule.
The AF2000 HT incorporates Postnova’s patented FOCUS technology, to ensure a continuous and stable detector flow at any time. The technology provides also a higher sample recovery, higher resolution and sensitivity.
Postnova is using a patented technology for generating the cross flow inside the FFF channel, based on a double syringe pump. This pump is controlled in a special unique way avoiding any pulsation.
The AF2000 MT series was developed to be used as Stand-Alone system or interfaced with existing HTGPC systems from various manufacturers. This gives the advantage that FFF and GPC can be performed using the same set or detectors, using the same temperature chamber for GPC and FFF.
The AF2000 AT is a non-temperature controlled economical Flow FFF which is ideal for the use in general level research in universities, companies and public scientific centers.
The AF2000 MT is a temperature controlled advanced Flow FFF system, which is ideal for the use in higher level research in university and public research institutions as well as professional research and commercial-industrial applications in the area of product development, manufacturing and quality control.
|Usage||To separate, fractionate, purify and to characterize microparticles in one step.|
|Brand Name||Gravitational Splitt FFF- SF2000 G|
The SF2000 G allows continuous separation and fractionation of microparticles by size and density. It is possible to collect mg to gram quantities and reach preparative scales.
The system is using a gravity field and thus can separate microparticles from about 1 to 300 μm in typical aqueous based carrier solutions. A resolution of 1 μm size difference can be achieved for smaller particles.
Because of the open channel and the absence of any stationary phase the separation in Splitt FFF can be performed under the absence of shear forces and stress avoiding particle aggregation.
The SF2000 G system can be easily hyphenated on- and offline to additional advanced detection systems, such as Absorbance (UV/Vis), Fluorescence (FLD), Dynamic Light Scattering (DLS), Optical Particle Detection (XPT), X-Ray, Laser Diffraction and Microscopy.
|Grade Standard||Technical Grade, Analytical Grade, Bio-Tech Grade|
|Physical State||Solid, Liquid, Granules|
The TF200 allows the simultaneous separation, characterization and fractionation of polymers, gels and particles within one measurement. The channel is reusable and only needs to be cleaned from time to time. There is no consumable which needs to be replaced frequently.
No size exclusion effect is observed, making the TF2000 the ideal tool for the separation of large and ultra-large cross-linked polymers.
Because of the open channel and the absence of any stationary phase the separation can be performed under the absence of shear forces and stress to the macromolecule. Typical chromatography problems, such as filtering/adsorption effects, shear induced chain degradation and late elution effects can be avoided.
Because of the absence of the stationary phase, nearly any kind of organic and even some aqueous liquids can be used in Thermal FFF. This allows to easily use also aggressive and otherwise difficult to use eluents such as DMSO for starch analysis etc. There is no need to add salt such as LiBr which is often essential in chromatography to avoid polymer interacting with stationary phases.
The TF2000 can be remote controlled, meets and conforms with the most stringent international security regulations ad offers series of automatic security features to allow unattended use. Temperature, pressure, leak and gas sensors are constantly monitoring the system for reproducibly and stable operation even in rough production environments.
The TF2000 series was developed to be used as Stand-Alone system or interfaced with existing chromatographic systems from various manufacturers.