Product Details:
Number of Reactions(Preps) per Kit | 100 |
Brand | Bio-Helix |
Result Time (Rapid Kits) | 30 minutes |
Method | PCR |
Usage/Application | PCR CleanUp and gel extraction |
Country of Origin | Made in India |
PureDireX PCR Clean-Up & Gel Extraction Kit
The PCR Clean-Up & Gel Extraction Kit provides a cost-effective system for the fast and easy isolation of the DNA fragments from PCR reactions, agarose gels, or enzymatic reactions. The DNA fragments (100bp~10Kb) in the special buffers are bound by the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure DNA is eluted with the Tris buffer or water without phenol extraction or alcohol precipitation. The DNA purified with the kits is suitable for any subsequent application, such as ligation and transformation, sequencing, restriction enzyme digestion, labeling, PCR, in vitro transcription, or microinjection. The entire procedure can be completed within 15~20 minutes.
Fastest Procedure: Completed within 30mins
Sample: up to 100μl of PCR Product or 300 mg of Agarose Gel
Recovery: up to 95%
Additional Information:
The DUAL Genomic DNA Isolation Kit (Blood/ Cultured Cell/ Fungus) combines the reagent system and spin column system. The kit is designed specifically for isolating the genomic DNA from the whole blood, frozen blood, buffy coat, cultured animal/ bacterial cells, and fungus. This unique reagent system ensures the total DNA with a high yield and good quality from the samples. The spin column system is designed to purify or concentrate DNA products which have been previously isolated with the reagents. The entire procedure can be completed in 1 hour without the phenol/ chloroform extraction. The purified DNA is suitable for use in PCR or other enzymatic reactions.
Sample
Up to 300 μl of the whole blood
Up to 200 μl of the frozen blood
Up to 200 μl of the buffy coat
Cultured animal cells (up to 1 x10^7)
Cultured bacterial cells (up to 1 x10^9)
Fungus cells (up to 5 x 10^7)
Format
Reagent and spin column
Yield
Up to 50 μg
Operation time
Within 60 minutes
Elution volume
50∼200 μl
Additional Information:
Additional Information: