Extraction And Purification Kits

Product Details:

PureDireX PCR Clean-Up & Gel Extraction Kit

The PCR Clean-Up & Gel Extraction Kit provides a cost-effective system for the fast and easy isolation of the DNA fragments from PCR reactions, agarose gels, or enzymatic reactions. The DNA fragments (100bp~10Kb) in the special buffers are bound by the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure DNA is eluted with the Tris buffer or water without phenol extraction or alcohol precipitation. The DNA purified with the kits is suitable for any subsequent application, such as ligation and transformation, sequencing, restriction enzyme digestion, labeling, PCR, in vitro transcription, or microinjection. The entire procedure can be completed within 15~20 minutes.

 

Fastest Procedure: Completed within 30mins
Sample: up to 100μl of PCR Product or 300 mg of Agarose Gel
Recovery: up to 95%

Additional Information:

• Item Code: PureDireX-PDC01-0100

The DUAL Genomic DNA Isolation Kit (Blood/ Cultured Cell/ Fungus) combines the reagent system and spin column system. The kit is designed specifically for isolating the genomic DNA from the whole blood, frozen blood, buffy coat, cultured animal/ bacterial cells, and fungus. This unique reagent system ensures the total DNA with a high yield and good quality from the samples. The spin column system is designed to purify or concentrate DNA products which have been previously isolated with the reagents. The entire procedure can be completed in 1 hour without the phenol/ chloroform extraction. The purified DNA is suitable for use in PCR or other enzymatic reactions.

Sample

Up to 300 μl of the whole blood
Up to 200 μl of the frozen blood
Up to 200 μl of the buffy coat
Cultured animal cells (up to 1 x10^7)
Cultured bacterial cells (up to 1 x10^9)
Fungus cells (up to 5 x 10^7)

Format
Reagent and spin column

Yield
Up to 50 μg

Operation time
Within 60 minutes

Elution volume
50∼200 μl

Additional Information:

• Item Code: PureDireX-PDC02-0100

The MBead Bacteria Genomic DNA Kit provides a fast, simple, and cost-effective method for isolating the genomic DNA from bacterial cells. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for DNA to be easily bound by the surface of the magnetic beads. The Phenol extraction and ethanol precipitation are not required, and the high-quality genomic DNA is eluted in the Release Buffer. The genomic DNA purified with the MBead Bacteria Genomic DNA Kit is suitable for a variety of routine applications. The entire procedure can be completed within 15-20 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

Additional Information:

• Item Code: PureDireX-PDM03-0100
• Packaging Details: 100 reactions